BUB1B anticorps (AA 883-1052)

N° du produit ABIN306786

Cet anticorps anti-BUB1B est un anticorps Mouton Polyclonal détectant BUB1B dans WB et IHC. Adapté pour Souris.

Aperçu rapide pour BUB1B anticorps (AA 883-1052) (ABIN306786)

Antigène: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivité: Souris

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp BUB1B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-BUB1B anticorps

Épitope: AA 883-1052

Réactivité croisée: Souris

Attributs du produit: Mitotic checkpoint kinase MAD3L,Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.

Purification: Ammonium Sulfate Precipitation

Immunogène: Recombinant protein corresponding to amino acids 883-1052 of the mouse Bub1b protein.

Isotype: IgG

Information d'application

Indications d'application: Antibody detects Bub1b in mitotic cells seperated from NIH/3T3 cells by IP/Westen blot (1-10 μg/mL) analysis. Detects a 130 kDa band. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur BUB1B

Antigène: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Autre désignation: Bub1 beta

UniProt: Q9Z1S0