Bub1 beta anticorps

N° du produit ABIN306788

L’anticorps anti-Bub1 beta Polyclonal Mouton est utilisé pour la détection de Bub1 beta dans des échantillons de Souris. Il a été validé pour IHC et WB.

Aperçu rapide pour Bub1 beta anticorps (ABIN306788)

Antigène: Bub1 beta

Reactivité: Souris

Hôte: Mouton

Clonalité: Polyclonal

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Détail du produit

Fonction: Sheep anti Mouse Bub1 beta-IN

Réactivité croisée: Souris

Attributs du produit: Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.

Purification: Ammonium Sulfate Precipitation

Isotype: IgG

Information d'application

Indications d'application: X1163P antibody detects Bub1b in mitotic NIH/3T3 cells, seperated by immunoprecipitation. Westen blot analysis (1-10 μg/mL) detects a 130 kDa band. Optimal antibody concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détail du antigène

Antigène: Bub1 beta

Sujet: Mitotic checkpoint kinase MAD3L

UniProt: Q9Z1S0