P34 Cdc-2 Nuclear Protein anticorps

N° du produit ABIN306818

L’anticorps anti-P34 Cdc-2 Nuclear Protein Monoclonal Souris est utilisé pour la détection de P34 Cdc-2 Nuclear Protein dans des échantillons de Boeuf (Vache), Humain, Souris, Rat et Xenopus laevis. Il a été validé pour ICC, IHC (p), IP et WB.

Aperçu rapide pour P34 Cdc-2 Nuclear Protein anticorps (ABIN306818)

Antigène: P34 Cdc-2 Nuclear Protein

Reactivité: Boeuf (Vache), Humain, Souris, Rat, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Application: Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Clone: POH-1

Détail du produit

Fonction: Mouse anti Human p34 cdc-2 nuclear protein

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat, Xenopus laevis

Attributs du produit: p34(Cdc2) kinase is a an essential nuclear protein for mitosis in human cells. During the prophase/metaphase transition the inactive phosphorylated form of p34(cdc2) is dephosphorylated to make the active form of the protein. This dephosphorylation is performed by the p80(cdc25) dual-specificity phosphatase. The regulatory subunit of p34(cdc2) is cyclin B, a 47-52 kDa protein. The p34(cdc2) protein is the catalytic subunit of the M phase-promoting factor (MPF) which is intracellular factor responsible for entry into M phase. Three mechanisms are responsible for the activitation of MPF at the onset of mitosis: binding of p34(cdc2) to cyclin B, a series of phosphorylation/dephosphorylation events on the two subunits and translocation of the complex to the nucleus or microtubules.

Purification: Protein A/G Chromatography

Immunogène: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant human p34.cdc2 fusion protein and mouse myeloma cells.

Isotype: IgG2a

Information d'application

Indications d'application: Detects three bands within the 34 kDa region corresponding to p34 and its cleavage products. Can also be used for IHC on paraffin-embedded tissue (10 μg/mL) as well as immunocytochemistry and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détail du antigène

Antigène: P34 Cdc-2 Nuclear Protein

UniProt: P06493