Kein 14 (C-Term) anticorps

N° du produit ABIN306820

Cet anticorps Souris Monoclonal détecte spécifiquement dans IHC (fro) et WB. Il présente une réactivité envers Humain.

Aperçu rapide pour Kein 14 (C-Term) anticorps (ABIN306820)

Antigène: Kein 14

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Clone: DE-SPK14

Détail du produit

Épitope: C-Term

Réactivité croisée: Humain

Attributs du produit: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. This antibody reacts specifically with human keratin 14 by immunoblotting. In tissue sections, it provides a positive reaction on basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingiva, myoepithelial cells and squamous cell carcinomas.

Purification: Protein A/G Chromatography

Immunogène: Hybridoma produced by the fusion of splenocytes from mice immunized with synthetic peptide conjugated to KLH corresponding to the carboxy terminal sequence of human cytokeratin 14 (KVVSTHEQVLRTKN) and mouse myeloma cells.

Isotype: IgG2b

Information d'application

Indications d'application: Detects human cytokeratin 14 by Western blot at 50 kDa. Does not react with other cytokeratins or other proteins. Also works for immunohistochemistry using frozen tissues. Does not work with paraffin embedded tissues. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détail du antigène

Antigène: Kein 14

UniProt: P02533