Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Réactivité croisée
Humain
Attributs du produit
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Cytokeratin 7 is a basic cytokeratin which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of the ovary, lung and breast but not colon or gastrointestinal tract.
Purification
Protein A/G Chromatography
Immunogène
Hybridoma produced by the fusion of splenocytes from mice immunized with purified human keratin 7 isolated from HeLa cytoskeleton and mouse myeloma cells.
Clone
C35
Isotype
IgG1
Indications d'application
Detects keratin 7 by Western blot. Detects a 54 kDa band corresponding to keratin 7. Optimal concentration should be evaluated by serial dilutions.
Restrictions
For Research Use only
Buffer
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles