MYOD1 anticorps

N° du produit ABIN306848

L’anticorps Souris Monoclonal anti-MYOD1 a été validé pour WB, ELISA, IF, IP, IHC (p) et IHC (fro). Il convient pour détecter MYOD1 dans des échantillons de Humain.

Aperçu rapide pour MYOD1 anticorps (ABIN306848)

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MYOD1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 5-2F

Détail du produit anti-MYOD1 anticorps

Réactivité croisée: Poulet, Humain, Souris, Porc, Rat

Attributs du produit: Myoblast determination protein 1, Myogenic factor 3, MyoD1, Myf-3,X1414M monoclonal antibody, clone 5.2F recognizes a protein of 45 kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Occassionally nuclear expression/staining of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining/presence is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas. The 5.2F antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue.

Purification: Protein A/G Chromatography

Immunogène: Recombinant mouse MyoD1 protein.,Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.

Isotype: IgG2a

Information d'application

Indications d'application: This antibody can be used for electron microscopy, ELISA, immunofluorescence, immunoprecipitation (2 μg/mg of protein lysate), Western blotting (1 μg/mL) and immunohistochemistry on frozen and formalin/paraffin fixed tissues (2-4 μg/mL). These working concentrations are merely an indication. Optimal working concentrations should, however, be evaluated by serial dilutions by the customer.

Restrictions: For Research Use only

Stockage

Buffer: Purified monoclonal antibody 5.2F in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur MYOD1

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Autre désignation: MyoD1

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development