Estrogen Receptor alpha anticorps (AA 302-595)

N° du produit ABIN306907

Cet anticorps Souris Monoclonal détecte spécifiquement Estrogen Receptor alpha dans WB, IP, ChIP, GS et InhA. Il présente une réactivité envers Humain.

Aperçu rapide pour Estrogen Receptor alpha anticorps (AA 302-595) (ABIN306907)

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Estrogen Receptor alpha est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Inhibition Assay (InhA)

Clone: TE111-5D11

Détail du produit anti-Estrogen Receptor alpha anticorps

Épitope: AA 302-595

Réactivité croisée: Poulet, Boeuf (Vache), Chien, Hamster, Humain, Souris, Porc, Lapin, Rat, Mouton

Attributs du produit: The estrogen receptor (ER) gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

Purification: Protein A/G Chromatography

Immunogène: Hybridoma produced by the fusion of splenocytes from mice immunized with the 35 kDa C-terminal fragment (aa 302-595) of human ER expressed in E. coli and mouse myeloma cells.

Isotype: IgG1

Information d'application

Indications d'application: This antibody can be used gel supershift assays (neat concentration), immunoprecipitation on native and denatured lysate (2 μg/mg of protein lysate), Western blotting (1-2 μg/mL) and chromatin precipitation. Can also be used to block binding of estrogen to the estrogen receptor. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur Estrogen Receptor alpha

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Autre désignation: Estrogen Receptor

UniProt: P11474

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly