HNRNPK anticorps (C-Term)

N° du produit ABIN307124

L’anticorps Souris Monoclonal anti-HNRNPK a été validé pour WB et IHC. Il convient pour détecter HNRNPK dans des échantillons de Humain.

Aperçu rapide pour HNRNPK anticorps (C-Term) (ABIN307124)

Antigène: HNRNPK (Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HNRNPK est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: F45 P9 C7

Détail du produit anti-HNRNPK anticorps

Épitope: C-Term

Réactivité croisée: Poulet, Humain, Souris, Lapin, Rat

Attributs du produit: hnRNP-K, CSBP, TUNP, dC-Stretch Binding Protein,Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA Polymerase II. There are approximately 20 known hnRNP proteins, which range in size from 34 kDa to 120 kDa, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus, however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65 kDa protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.

Purification: Protein A/G Chromatography

Immunogène: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminal of the hnRNP-K protein and mouse myeloma Ag8563 cells. Sequence common in human, mouse, rat, rabbit and chicken

Isotype: IgG1

Information d'application

Indications d'application: Antibody can be used for Western blotting (1-2 μg/mL) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 μg/mL). Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur HNRNPK

Antigène: HNRNPK (Heterogeneous Nuclear Ribonucleoprotein K (HNRNPK))

Autre désignation: Heterogeneous Nuclear Ribonucleoprotein K

UniProt: P61978