GAL-4, DNA Binding Domain (full length) anticorps

N° du produit ABIN307169

L’anticorps anti-GAL-4, DNA Binding Domain Monoclonal Souris est utilisé pour la détection de GAL-4, DNA Binding Domain dans des échantillons de Saccharomyces cerevisiae. Il a été validé pour WB et IP.

Aperçu rapide pour GAL-4, DNA Binding Domain (full length) anticorps (ABIN307169)

Antigène: GAL-4, DNA Binding Domain

Reactivité: Saccharomyces cerevisiae

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 8C-1

Détail du produit

Épitope: full length

Fonction: Mouse anti Yeast GAL-4 DNA Binding Domain

Réactivité croisée: Saccharomyces cerevisiae

Attributs du produit: Binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

Purification: Protein A/G Chromatography

Immunogène: Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.

Isotype: IgG1

Information d'application

Indications d'application: Recognizes a 106 kDa protein by Western blot in yeast cell extracts corresponding to amino acids 1-147 of the GAL4 DNA binding region. Western blot at 1-10 μg/mL. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détail du antigène

Antigène: GAL-4, DNA Binding Domain

Autre désignation: GAL-4 DNA Binding Domain

Sujet: Mouse anti GAL-4 DNA Binding region, gal4, gal 4 yeast