5-Methylcytidine anticorps

N° du produit ABIN308218

Détail du produit anti-5-Methylcytidine anticorps

Antigène: 5-Methylcytidine

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp 5-Methylcytidine est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Specificité: Modified base 5-methylcytidine

Purification: Purified IgG from culture supernatant by Affinity chromatography on Protein A ceramic hyper D F - Filtration on 0.22 µm.

Clone: 33D3

Isotype: IgG1

Information d'application

Indications d'application: MeDIP: Used 5ul in 200ul reaction with 2ug digested genomic DNA from Drosophila. Flow Cyt: Use 10ul of working dilution to label 1000000 cells in 100ul. ICC/IF: Used at a dilution of 1/200 for 16 hrs. IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium citrate buffer pH6.0 is recommended for this purpose. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

Commentaires:

For research use only

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/ml

Buffer: Phosphate Buffered Saline 10 mM – NaCl 0.15 M – pH 7.4

Stock: -20 °C

Références pour anticorps anti-5-Methylcytidine (ABIN308218)

Ohta, Sakaide, Wakayama: "Functional analysis of male mouse haploid germ cells of various differentiation stages: early and late round spermatids are functionally equivalent in producing progeny." dans: Biology of reproduction, Vol. 80, Issue 3, pp. 511-7, (2009) (PubMed).

Xing, Shi, Le, Lee, Silver-Morse, Li: "Evidence for transgenerational transmission of epigenetic tumor susceptibility in Drosophila." dans: PLoS genetics, Vol. 3, Issue 9, pp. 1598-606, (2007) (PubMed).

Détails sur 5-Methylcytidine

Antigène: 5-Methylcytidine

Abstract: 5-Methylcytidine Produits

Classe de substances: Chemical

Sujet: DNA methylation is a DNA modification process, which is involved in the control of gene expression. The 5-Methylcytidine antibody specifically binds the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates.