RYR1 anticorps (C-Term)

N° du produit ABIN317452

Cet anticorps Souris Monoclonal détecte spécifiquement RYR1 dans WB, IF, EIA, IHC (fro) et FACS. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Chien et Porc.

Aperçu rapide pour RYR1 anticorps (C-Term) (ABIN317452)

Antigène: RYR1 (Ryanodine Receptor 1 (Skeletal) (RYR1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RYR1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Clone: G-1

Détail du produit anti-RYR1 anticorps

Épitope: C-Term

Specificité: This antibody reacts with the C-terminus cytoplasmic domain of Ryanodine Receptor. Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscle cells. This antibody reacts with Ryanodine Receptor (~500 kDa) isolated from a variety of cell types (e.g. lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscle, cardiac muscle and brain tissues).

Purification: Purified

Immunogène: Short synthetic polypeptide corresponding to the C-terminal domain of the ryanodinereceptor

Isotype: IgG2b

Information d'application

Indications d'application: Immunohistochemistry on frozen sections: 1: 100 (Use indirect immunofluorescence. Visualization by confocal microscopy is required, as detection by standard fluorescentmicroscopy will not be adequate to detect the RyR. Additionally, fluorescent, notenzymatic, detection is required. Due to the intensity of confocal lasers, use of ananti-fading agent, such as DABCO, is strongly recommended. ). Immunofluorescence: 1: 100. ELISA: 1: 1000. Flow Cytometry: 1: 500. Western blot: 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate werecommend microcentrifugation before use.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur RYR1

Antigène: RYR1 (Ryanodine Receptor 1 (Skeletal) (RYR1))

Autre désignation: Ryanodine Receptor 1 / RYR1

ID gène: 6261

UniProt: P21817

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development