EIF3E anticorps
Aperçu rapide pour EIF3E anticorps (ABIN317905)
Antigène
Voir toutes EIF3E AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- EIF3ε antibody detects endogenous levels of eIF3ε protein.(region surrounding Val116)
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Purification
- Affinity chromatography
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Indications d'application
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ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1,0 mg/mL
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Buffer
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))
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Autre désignation
- EIF3E / EIF3S6
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Sujet
- Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Eukaryotic initiation factor 3 (eIF3) is the largest family of eIFs and consists of at least 12 unique subunits in mammals. eIFε, also known as eIF p47, binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA and associates with the complex p170-eIF3.Synonyms: Eukaryotic translation initiation factor 3 subunit 6, Eukaryotic translation initiation factor 3 subunit E, INT6, Viral integration site protein INT-6 homolog, eIF-3 p48
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Poids moléculaire
- approx. 38 kDa
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ID gène
- 3646
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NCBI Accession
- NP_001559
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UniProt
- P60228
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Pathways
- Ribonucleoprotein Complex Subunit Organization, Hepatitis C
Antigène
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