PDCD4 anticorps (C-Term)

N° du produit ABIN318336

Cet anticorps anti-PDCD4 Polyclonal Lapin (ABIN318336) détecte spécifiquement PDCD4 dans WB, IHC (p), EIA et IHC (fro). L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Xenopus laevis et Staphylococcus aureus.

Aperçu rapide pour PDCD4 anticorps (C-Term) (ABIN318336)

Antigène: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reactivité: Humain, Souris, Rat, Xenopus laevis, Staphylococcus aureus

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PDCD4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-PDCD4 anticorps

Épitope: C-Term

Specificité: The antibody reacts with Pdcd4 protein that is either phosphorylated or non-phosphorylated at Ser457.

Réactivité croisée (Details): Species reactivity (expected):A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from Human, Mouse, Rat and Xenopus based on 100 % homology with the immunizing sequence.
Species reactivity (tested):Human, Mouse, Rat, S. aureus, Xenopus.

Purification: Affinity Chromatography.

Immunogène: Synthetic peptide corresponding amino acids near the carboxyl terminus of Human Pdcd4 protein.

Information d'application

Indications d'application: ELISA: 1/10,000-1/50,000. Western blot: 1/1,000-1/10,000 (a band approximately 52 kDa in size corresponding toPdcd4 protein in the appropriate cell lysate or extract). Immunohistochemistry: 1: 100-1/300.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.06 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PDCD4

Antigène: PDCD4 (Programmed Cell Death 4 (PDCD4))

Autre désignation: PDCD4

Sujet: Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4

ID gène: 27250

NCBI Accession: NP_055271

UniProt: Q53EL6