Cathepsin G anticorps (Internal Region)

N° du produit ABIN3183694

Cet validé indépendamment anticorps Lapin Polyclonal détecte spécifiquement Cathepsin G dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour Cathepsin G anticorps (Internal Region) (ABIN3183694)

Antigène: Cathepsin G (CTSG)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cathepsin G est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-Cathepsin G anticorps

Épitope: Internal Region

Specificité: Cathepsin G Polyclonal Antibody detects endogenous levels of Cathepsin G protein.

Attributs du produit: Rabbit Polyclonal to Cathepsin G.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from the Internal region of human Cathepsin G.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, ELISA 1:40000,

Restrictions: For Research Use only

Independent Validation (UP)

Validation #102721 (Unfolding Profile)

by: NanoTemper Technologies

No.: #102721

Date: 05.11.2018

Antigène: CTSG

Numéro du lot: 022204892

Application validée: Unfolding Profile

Contrôle positif: ABIN3183694

Conclusion:

Passed. ABIN3183694 showed T_i at 85.2°C and a clear unfolding profile with one unfolding event. This suggests that the protein is properly folded and functional.

Validation Images

p>Unfolding profile of ABIN3183694. The fluorescence signal is plotted against temperature. The native (folded) protein has a high signal at the beginning of the experiment, which decreases and then again increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 85.2°C.

Protocole

Anticorps primaire: ABIN3183694

Full Protocol:

• Dilute ABIN3183694 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 0.67µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Notes:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Store at -20°C.

Détails sur Cathepsin G

Antigène: Cathepsin G (CTSG)

Autre désignation: Cathepsin G

Poids moléculaire: 22 kDa

ID gène: 1511

UniProt: P08311

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones