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HA-Tag anticorps

L’anticorps Lapin Polyclonal anti-HA-Tag a été validé pour WB, EIA et IHC (p). Il convient pour détecter HA-Tag dans des échantillons de .
N° du produit ABIN318728

Aperçu rapide pour HA-Tag anticorps (ABIN318728)

Antigène

HA-Tag

Reactivité

Veuillez nous consulter SVP

Hôte

  • 86
  • 86
  • 13
  • 9
  • 1
Lapin

Clonalité

  • 98
  • 93
  • 1
Polyclonal

Conjugué

  • 80
  • 15
  • 15
  • 9
  • 3
  • 3
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  • 3
  • 2
  • 2
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  • 2
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  • 2
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  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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Cet anticorp HA-Tag est non-conjugé

Application

  • 150
  • 62
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  • 33
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  • 9
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Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Séquence

    YPYDVPDYA

    Purification

    Purified

    Immunogène

    Keyhole limpet hemocyanin conjugated epitope tag peptide (114-122) from haemagglutinin influenza. A cysteine residue was used to facilitate coupling at the C-terminal end.

    Isotype

    IgG
  • Indications d'application

    Immunohistochmemistry on paraffin sections (Requires antigen retrieval using heattreatment prior to staining of paraffin embedded sections. Citrate buffer is recommendedfor this purpose). ELISA. Western blot.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    0.02 M Potassium phosphate, 0.12 M Sodium chloride pH 7.2, 0.01 % Sodium Azide (NaN3)

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
  • Antigène

    HA-Tag

    Autre désignation

    HA Epitope Tag (YPYDVPDYA)

    Classe de substances

    Tag

    Sujet

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
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