CD84 anticorps (Internal Region)

N° du produit ABIN3187786

Cet anticorps anti-CD84 est un anticorps Lapin Polyclonal détectant CD84 dans WB, ELISA et IHC (p). Adapté pour Humain et Souris. Validé indépendamment pour une utilisation dans Immunohistochemistry.

Aperçu rapide pour CD84 anticorps (Internal Region) (ABIN3187786)

Antigène: CD84

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD84 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-CD84 anticorps

Épitope: Internal Region

Specificité: CD84 Polyclonal Antibody detects endogenous levels of CD84 protein.

Attributs du produit: Rabbit Polyclonal to CD84.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from the Internal region of human CD84.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC-P 1:100-300, ELISA 1:20000,

Restrictions: For Research Use only

Independent Validation (IHC)

Validation #104537 (Immunohistochemistry)

by: TRON

No.: #104537

Date: 07.12.2023

Antigène: CD84

Numéro du lot: 15601601

Application validée: Immunohistochemistry

Contrôle positif:

FFPE human tonsil

Conclusion:

Passed. The CD84 antibody ABIN3187786 specifically stains cells within the germinal zone in human tonsil.

Validation Images

IHC staining of human tonsil with anti-CD84 antibody ABIN3187786 (1:100) and counterstain with hematoxylin.

IHC staining of human tonsil with anti-PD-1 antibody ABIN3187786 (1:200) and counterstain with hematoxylin.

Protocole

Anticorps primaire: ABIN3187786

Anticorps secondaire: HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122)

Full Protocol:

• Dewax and rehydrate tissue sections on glass slides in a descending xylene and graded alcohol series:
• • xylene 2x for 5 min.
  • 100 % (v/v) ethanol 2x for 5 min.
  • 96 % (v/v) ethanol 2x for 5 min.
  • 70 % (v/v) ethanol 2x for 5 min.
• Wash slides 2x 5 min with ddH2O.
• Cook slides for 30 min at 95 °C in citrate buffer pH 6.
• Wash slides 2x 3 min with ddH2O.
• Incubate slides in 0.3 % (v/v) H2O2 in PBS for 15 min to quench endogenous peroxidase.
• Wash slides 3x 3 min in 1X PBS.
• Block slides in 10 % goat serum in PBS for 30 min at RT.
• Incubate slides with primary rabbit anti-CD84 antibody (antibodies-online, ABIN3187786, lot 15601601) diluted 1:100 and 1:200 in 10 % goat serum in PBS ON at 4 °C in a humidified chamber.
• Wash slides 3x 3 min in 1X PBS.
• Incubate slides with secondary HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122) ready-to-use in 10 % goat serum in PBS for 30 min at RT in a humidified chamber.
• Wash slides 3x 3 min in 1X PBS.
• HRP development.
• Counterstain slides with hematoxylin solution (Carl Roth, T865.2).
• Wash slides for 15 min under running tap water.
• Dehydrate sections in an ascending alcohol-xylene series:
• • 70% ethanol 2x for 10 sec.
  • 96% ethanol 2x for 10 sec.
  • 100% ethanol 2x for 10 sec.
  • xylene 2x for 10 sec.
• Mount sections with Entellan new mounting medium (Sigma-Aldrich, 1008690500).
• Dry sections.
• Image acquisition on an Aperio CS2 Digital Pathology Slide Scanner (Leica), Magnification 40x.

Notes:

• Staining with ABIN3187786 overnight at 1:100 and 1:200 antibody dilution the signal in human tonsil was sharp and localized in the cells within the germinal zone (as expected). Modifying ABIN3187786 incubation conditions to 1 h at RT, the signal in human tonsil was still sharp and localized in the cells within the germinal zone.

• However, in mouse spleen, the signal was almost absent and very weak. With the modified incubation conditions, the signal in mouse spleen was again almost absent and very weak. With either staining protocol the signal in mouse lung was sporadic and its quality does not allow the possibility of non-specific staining to be excluded.

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Store at -20°C.

Détails sur CD84

Antigène: CD84

Autre désignation: CD84

Poids moléculaire: 39 kDa

ID gène: 8832

UniProt: Q9UIB8