PDCD4 anticorps (C-Term)

N° du produit ABIN318876

Cet anticorps anti-PDCD4 Polyclonal Lapin (ABIN318876) détecte spécifiquement PDCD4 dans WB et EIA. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat et Xenopus laevis.

Aperçu rapide pour PDCD4 anticorps (C-Term) (ABIN318876)

Antigène: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reactivité: Humain, Souris, Rat, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PDCD4 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-PDCD4 anticorps

Épitope: C-Term

Specificité: This antibody detects a specific epitope within the carboxy-terminal of programmed cell death 4 (Pdcd4), a novel tumour suppressor.

Purification: Purified

Immunogène: Synthetic peptide corresponding to amino acids near the carboxy terminal of human Pdcd4.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry on paraffin sections: 1: 100 - 1: 300, requires protein digestionpretreatment of paraffin sections e. g. trypsin or pronase. ELISA: 1: 10000 - 1: 50000. Western Blot: 1: 1000 - 1: 10000, detects a band of approximately 52 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.01 % Sodium Azide (NaN3)

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Stock: 4 °C

Stockage commentaire: Store at +4 °C or at -20 °C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted.

Détails sur PDCD4

Antigène: PDCD4 (Programmed Cell Death 4 (PDCD4))

Autre désignation: PDCD4

Sujet: Pdcd4 interacts with the RNA helicase eIF4A and the scaffolding protein eIF4G, two components of the translation initiation complex, and thus inhibits protein synthesis. Pdcd4 has also been shown to inhibit transcription factor activator protein-1 (AP-1), which regulates several events required for cell invasion, including expression of matrix metalloproteinases and cell motility. Pdcd4 localizes to the nucleus in proliferating cells, and its expression is modulated by cytokines in natural killer and T cells. Pdcd4 expression is down regulated with tumour progression in carcinomas of the lung, colon, prostate and breast.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4

ID gène: 27250

NCBI Accession: NP_055271

UniProt: Q53EL6