Selectin E/CD62e anticorps

N° du produit ABIN319451

L’anticorps Souris Monoclonal anti-Selectin E/CD62e a été validé pour WB, IP, FACS, IHC (fro) et EIA. Il convient pour détecter Selectin E/CD62e dans des échantillons de Humain et Porc.

Aperçu rapide pour Selectin E/CD62e anticorps (ABIN319451)

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivité: Humain, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Selectin E/CD62e est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 1-2B6

Détail du produit anti-Selectin E/CD62e anticorps

Specificité: This antibody recognises the CD62E and CD62P cell surface antigens. MCA883 inhibits neutrophil adhesion to E-selectin and to P-selectin. Although previously thought to recognise only human CD62E, recent data (5) shows that this antibody also recognises human CD62P binding to a common epitope on these members of the selectin family.

Réactivité croisée (Details): Also reacts to: CD62P

Purification: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Immunogène: Human E-Selectin (ELAM-1)

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on frozen sections: 1g/mL - 10g/mL. Positive Control: Human Tonsil. ELISA: 10g/mL. Western Blot: Non-reduced only, 10l of the suggested working dilution to label 10^6 cellsin 100 μL. Flow Cytometry: 10g/mL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline containing 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Selectin E/CD62e

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Autre désignation: CD62E / E-Selectin

Sujet: CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.Synonyms: CD62 antigen-like family member E, ELAM1, Endothelial leukocyte adhesion molecule 1, LECAM2, Leukocyte-endothelial cell adhesion molecule 2, SELE

ID gène: 6401

NCBI Accession: NP_000441

UniProt: P16109

Pathways: Thromboxane A2 Receptor Signaling