CD8 anticorps

N° du produit ABIN319569

Cet anticorps anti-CD8 est un anticorps Rat Monoclonal détectant CD8 dans FACS et IHC (fro). Adapté pour Souris.

Aperçu rapide pour CD8 anticorps (ABIN319569)

Antigène: CD8

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: YTS105-18

Détail du produit anti-CD8 anticorps

Specificité: SM024LE recognises a non polymorphic epitope on the mouse CD8 alpha chain. Antibodies produced by clone YTS105.18 have been reported to block MHC I dependent T-cell responses in vitro and in vivo, and induce transplantation tolerance in combination with CD4 antibodies.

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

niveau d'endotoxine: Low Endotoxin

Immunogène: Mouse spleen cells. Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3. myeloma cell line.

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur CD8

Antigène: CD8

Autre désignation: CD8

Sujet: CD8 T cell surface antigen is heterodimer of an alpha and a beta chain linked by two disulfide bonds. It belongs type I membrane protein. Selectively expressing of CD8 on a subset of T cells leads to CD8 T cell development. Through identifying cytotoxic/suppressor T-cells that interact with MHC class I bearing targets, CD8 is thought to play a role in the process of T-cell mediated killing. Veillette et al (1988) found the CD8 is associated with the internal membrane tyrosine-protein kinase p56lck.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

ID gène: 12525

NCBI Accession: NP_001074579

UniProt: P01731