Isocitrate Dehydrogenase anticorps

N° du produit ABIN3197487

Cet anticorps Lapin Polyclonal détecte spécifiquement Isocitrate Dehydrogenase dans WB et IF. Il présente une réactivité avec des échantillons de Cabbage, Zea mays, Tomato, Soybean, Red pepper, Potato, Pea, Oryza sativa, Nicotiana benthamiana et Arabidopsis thaliana. Il a été cité dans 3+ publications.

Aperçu rapide pour Isocitrate Dehydrogenase anticorps (ABIN3197487)

Antigène: Isocitrate Dehydrogenase (IDH)

Reactivité: Cabbage, Zea mays, Tomato, Soybean, Red pepper, Potato, Pea, Oryza sativa, Nicotiana benthamiana, Arabidopsis thaliana

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Isocitrate Dehydrogenase est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-Isocitrate Dehydrogenase anticorps

Fonction: Anti-IDH | Isocitrate dehydrogenase (Cellular [compartment marker] of mitochondrial matrix)

Réactivité croisée (Details): Arabidopsis thaliana, Brassica oleracea, Capsicum annuum, Glycine max, Lycopersicum chilense, Nicotiana benthamiana, Oryza sativa, Solanum lycopersicum, Pisum sativum, Solanum sogarandium, Solanum tuberosum, Zea mays
Not reactive in: Chlamydomonas reinhardtii

Homologie: Brachypodium distachyon,Brassica napus,Capsella rubella,Citrus sinensis,Hordeum vulgare,Malus x domestica,Medicago truncatula,Nicotiana tabacum,Phaseolus vulgaris,Theobroma cacao,Triticum aestivum,Vitis vinifera,Zea mays

Purification: Immunogen affinity purified serum

Immunogène: KLH-conjugated peptide 1 and peptide 2 conserved in all higher plants mitochondrial

Information d'application

Indications d'application: 1:400 (IF), 1 : 5 000 (WB)

Commentaires:

cellular [compartment marker] of mitochondrial matrix

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Références pour anticorps anti-Isocitrate Dehydrogenase (ABIN3197487)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." dans: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Lee, Lee, Yoo, Duncan, Oh, Lee, Lee, Whelan, Hwang: "Mitochondrial targeting of the Arabidopsis F1-ATPase γ-subunit via multiple compensatory and synergistic presequence motifs." dans: The Plant cell, Vol. 24, Issue 12, pp. 5037-57, (2013) (PubMed).

Szal, Jastrz?bska, Kulka, Lea: "Influence of mitochondrial genome rearrangement on cucumber leaf carbon and nitrogen metabolism." dans: Planta, Vol. 232, Issue 6, pp. 1371-82, (2010) (PubMed).

Détails sur Isocitrate Dehydrogenase

Antigène: Isocitrate Dehydrogenase (IDH)

Sujet:

Background: Plant NADH dependent isocitrate dehydrogenase enzyme is located in mitochondrial matrix. This enzyme is classified as an oxidoreductase and its function is to catalyze a reaction in the citric acid cycle, specifically the sequential dehydrogenation and decarboxylation of isocitrate to form a-ketoglutarate. It removes hydrogens from its substrate, isocitrate. In addition to this process, it functions as a decarboxylase, removing a CO2 from the six-carbon substrate to form a five-carbon product mentioned above as a-ketoglutarate. There are two forms of this enzyme NADP+ and NAD+ dependent.

Additional information: Peptide used to elicit this antibody is not conserved in NADPH dependent anzymes, partially conserved across eukaryotic Idh subunits, Some conservation across bacterial which contain the NAD-dependent form of Idh (as opposed to the NADP-dependent form)

Poids moléculaire: 39 | 45 kDa (Arabidopsis thaliana)

ID gène: 816218

UniProt: P93032