Ribonucleotide Reductase Inhibiting Protein SML1 anticorps

N° du produit ABIN3197499

Détail du produit

Antigène: Ribonucleotide Reductase Inhibiting Protein SML1

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Application: Western Blotting (WB)

Réactivité croisée (Details): Not reactive in: no confirmed exceptions from predicted reactivity known at the moment

Homologie: Saccharomyces cerevisiae

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 11.83 / 11-12 kDa

Purification: serum

Immunogène: KLH-conjugated synthetic peptide derived from known S.cerevisie Sml1 sequence. Gene ID: 854945

Information d'application

Indications d'application: 1 : 1000 with standard ECL (WB)

Commentaires:

Cell preparation for western blot: cells were harvested by centrifugation (4000 x g , 6 min, 4 °C). Supernatant was discarded and cells were resuspended in 500 µl cold TCA buffer (20 mM Tris, pH 8, 50 mM ammonium acetate, 2 mM EDTA, 1 tablet/10 ml of Complete Mini Protease inhibitor cocktail with EDTA (Roche Diagnostics GmbH)). 500 µl 0.5 mm Zirconia/Silica Beads (BioSpec Products, Inc. 11079105z) and 500 µl cold 20 % TCA was added. Samples were vigorously vortexed twice for 30 sec (kept on ice in between). 750 µl from the liquid phase was transferred into a fresh Eppendorf tube. Samples were centrifuged for 10 min (20000 x g, 4 °C). The pellet was resuspended in 300 µl TCA-Laemmli buffer and boiled for 10 min at 100 °C.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Référence

Trump et al.: "Commentary on: "Enzalutamide monotherapy in hormone-naive prostate cancer: primary analysis of an open-label, single-arm, phase 2 study." Tombal B, Borre M, Rathenborg P, Werbrouck P, Van Poppel H, ..." dans: Urologic oncology, (2015) (PubMed).

Yoshida, Bacal, Desmarais, Padioleau, Tsaponina, Chabes, Pantesco, Dubois, Parrinello, Skrzypczak, Ginalski, Lengronne, Pasero: "The histone deacetylases sir2 and rpd3 act on ribosomal DNA to control the replication program in budding yeast." dans: Molecular cell, Vol. 54, Issue 4, pp. 691-7, (2014) (PubMed).

Détail du antigène

Antigène: Ribonucleotide Reductase Inhibiting Protein SML1

Sujet: Sml1 is a ribonucleotide reductase inhibitor involved in regulation of dNTP production. It is regulated by Mec1p and Rad53p during DNA damage and S phase. Synonymes: Yml058w.

Poids moléculaire: expected: 11.83 kDa, apparent: 11-12 kDa

ID gène: 854945