Fibroblasts, Epithelial Cells anticorps (PE)

N° du produit ABIN319868

Il existe 2+ publications pour ce produit. L’anticorps anti-Fibroblasts, Epithelial Cells Monoclonal Souris est utilisé pour la détection de Fibroblasts, Epithelial Cells dans des échantillons de Humain. Il a été validé pour FACS.

Aperçu rapide pour Fibroblasts, Epithelial Cells anticorps (PE) (ABIN319868)

Antigène: Fibroblasts, Epithelial Cells

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: PE

Application: Flow Cytometry (FACS)

Clone: D7-FIB

Détail du produit

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogène: Human foreskin Fibroblasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.25 mL (SM1214RT) or 1 mL (SM1214R) distilled water.

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA, 5 % Sucrose

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Référence

Raaijmakers, Widmer, Narechania, Eichhoff, Freiberger, Wenzina, Cheng, Mihic-Probst, Desalle, Dummer, Levesque: "Co-existence of BRAF and NRAS driver mutations in the same melanoma cells results in heterogeneity of targeted therapy resistance." dans: Oncotarget, Vol. 7, Issue 47, pp. 77163-77174, (2018) (PubMed).

Nazareth, Broderick, Simpson-Abelson, Kelleher, Yokota, Bankert: "Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 9, pp. 5552-62, (2007) (PubMed).

Détail du antigène

Antigène: Fibroblasts, Epithelial Cells

Autre désignation: Fibroplasts / Epithelial Cells

Sujet: A fibroblast is a connective-tissue cell of mesenchymal (somewhat undifferentiated) origin that secretes proteins from which the extracellular fibrillar matrix of connective tissue forms. Epithelial cells are cells that cover the surface of the body and line its cavities.Synonyms: Fibroblast Marker, Fibroblasten