Cet anticorps anti-Histiocytes Monoclonal Souris (Clone 3A5) (ABIN319872) détecte spécifiquement Histiocytes dans IHC (fro) et IHC (p).
L’anticorps est réactif avec des échantillons de Humain.
This antibody recognises an antigen expressed by human macrophages, monocytes and histiocytes, but does not stain interdigitating dendritic cells, Langerhans cells, microglial cells, or lymphoid cells. Some staining of basophilic granulocytes and of mast cells may be seen. In immunohistochemical studies the antibody shows partial cross-blocking with some anti-CD68 monoclonal antibodies, suggesting that the epitope may be associated with this antigen.
Purification
Tissue Culture Supernatant serum free
Immunogène
Human spleen cell homogenate, depleted of lymphocytes and erythrocytes
Isotype
IgG2b
Indications d'application
Immunohistochemistry on Frozen and Paraffin Sections: 1/10-1/100, This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Contol: Spleen. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Buffer
Contains 0.09 % Sodium Azide as preservative and 0.7 % BSA as stabilizer.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.