SLA (Haploid Sperm Cell-Specific Antigen) anticorps

N° du produit ABIN3201019

Il existe 3+ publications pour ce produit. L’anticorps anti-SLA (Haploid Sperm Cell-Specific Antigen) Monoclonal Rat est utilisé pour la détection de SLA (Haploid Sperm Cell-Specific Antigen) dans des échantillons de Souris et Rat. Il a été validé pour IEM, IHC et WB.

Aperçu rapide pour SLA (Haploid Sperm Cell-Specific Antigen) anticorps (ABIN3201019)

Antigène: SLA (Haploid Sperm Cell-Specific Antigen)

Reactivité: Souris, Rat

Hôte: Rat

Clonalité: Monoclonal

Application: Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC), Western Blotting (WB)

Clone: TRA54

Détail du produit

Réactivité croisée (Details): Not tested in other species.

Attributs du produit: The antibody was purified from the serum-free culture medium of the hybridoma clone TRA54 by proprietary chromatography processes under mild conditions.
This antibody can be used for immunohistochemical staining of testicular tissues. The hybridoma producing TRA54 was obtained from the immunaization of a rat with cell lysates of adult mouse testes by the group of Prof. Yoshitake Nishimune of Osaka University.

Purification: Purified

Isotype: IgM

Information d'application

Indications d'application: 1. Western blotting: ~1 g/mL
2. Immunohistochemical staining
3. Immunoelectron microscopy
Not tested in other applications

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 50 % Glycerol

Agent conservateur: Azide free

Stock: -20 °C

Référence

Arrotéia, Joazeiro, Yamada, Tanaka, Nishimune, Pereira: "Identification and characterization of an antigen recognized by monoclonal antibody TRA 54 in mouse epididymis and vas deferens." dans: Journal of andrology, Vol. 25, Issue 6, pp. 914-21, (2004) (PubMed).

Ventelä, Toppari, Parvinen: "Intercellular organelle traffic through cytoplasmic bridges in early spermatids of the rat: mechanisms of haploid gene product sharing." dans: Molecular biology of the cell, Vol. 14, Issue 7, pp. 2768-80, (2003) (PubMed).

Pereira, Tanaka, Nagata, Sawada, Mori, Chimelli, Nishimune: "Characterization and expression of a stage specific antigen by monoclonal antibody TRA 54 in testicular germ cells." dans: International journal of andrology, Vol. 21, Issue 1, pp. 34-40, (1998) (PubMed).

Détail du antigène

Antigène: SLA (Haploid Sperm Cell-Specific Antigen)

Sujet: Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organelles in germ cell cytoplasm from spermatocytes to spermatids. Western blots using TRA54 revealed broad bands with 85 kD, 190 kD and >200 kDa in testis probably due to alternative splicing and/or post-translational modification. The molecular size diversity is due to the difference in glycosilation.