PD-1 anticorps (Extracellular Domain)

N° du produit ABIN325052

L’anticorps Chèvre Polyclonal anti-PD-1 a été validé pour FACS, WB, IHC et ELISA. Il convient pour détecter PD-1 dans des échantillons de Souris.

Aperçu rapide pour PD-1 anticorps (Extracellular Domain) (ABIN325052)

Antigène: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PD-1 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-PD-1 anticorps

Épitope: Extracellular Domain

Specificité: Recognizes mouse PD-1 extracellular domain. Shows approximately 50 % cross-reactivity with rhPD-1.

Purification: Immunoaffinity purified

Immunogène: Sf21-derived rmPD-1 extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, Flo, IHC, WB

Usage: Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse PD-1. The detection limit for rmPD-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody will detect PD-1 in cells and tissues. The working dilution is 2-15 μg/mL. Flow Cytometry: This antibody can be used at 3-10 μg/mL/1 x10⁶ with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse PD-1. The detection limit for rmPD-1 is approximately 0.03 ng/well.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.5 mL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Conseil sur la manipulation: Avoid frequent freeze thaws. Store undiluted.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur PD-1

Antigène: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Autre désignation: PDCD1 / CD279 / PD-1

Sujet: Name/Gene ID: PDCD1
Family: Apoptosis

Synonyms: PDCD1, CD279, HPD-1, HPD-l, PD1, Protein PD-1, SLEB2, PD-1, CD279 antigen, Programmed cell death 1

ID gène: 5133

UniProt: Q15116

Pathways: Cancer Immune Checkpoints