IL17F anticorps (AA 31-163)

N° du produit ABIN327416

L’anticorps Souris Monoclonal anti-IL17F a été validé pour ELISA et FACS. Il convient pour détecter IL17F dans des échantillons de Humain.

Aperçu rapide pour IL17F anticorps (AA 31-163) (ABIN327416)

Antigène: IL17F (Interleukin 17F (IL17F))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL17F est non-conjugé

Application: ELISA, Flow Cytometry (FACS)

Clone: 197301

Détail du produit anti-IL17F anticorps

Épitope: AA 31-163

Specificité: Recognizes human IL-17F. In direct ELISAs and Western Blots this antibody does not cross-react with rhIL-17A, B, C, D, or E.

Purification: Protein G purified

Immunogène: E. coli-derived rhIL-17F corresponding to aa 31-163 according to Q96PD4.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Information d'application

Indications d'application: Approved: ELISA, Flo

Usage: Suitable for use in ELISA and Flow Cytometry. The detection limit is ~25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry (Intracellular): 25 μg/mL. Add 10 μL to 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. For intracellular staining to detect IL-17F, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. This antibody was tested using PMA and Ca2+ ionomycin-treated PBMCs. ELISA (Direct): 0.5-1.0 μg/mL. The detection limit is ~1 ng/well.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 200 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: -20 °C

Stockage commentaire: Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.

Détails sur IL17F

Antigène: IL17F (Interleukin 17F (IL17F))

Autre désignation: IL17F

Sujet: Name/Gene ID: IL17F

Synonyms: IL17F, CANDF6, Cytokine ML-1, IL-17F, Interleukin-17F, Interleukin 17F, Interleukin-24, ML-1, ML1, Mutant IL-17F

ID gène: 112744

UniProt: Q96PD4

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity