IL17D anticorps (AA 25-205)
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- Antigène Voir toutes IL17D Anticorps
- IL17D (Interleukin 17D (IL17D))
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Épitope
- AA 25-205
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Reactivité
- Souris
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Hôte
- Rat
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp IL17D est non-conjugé
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Application
- ELISA, Western Blotting (WB), Flow Cytometry (FACS)
- Specificité
- Recognizes mouse IL-17D. In direct ELISAs and Western Blots this antibody shows no cross-reactivity with rhIL-17D, rmIL-17, rmIL-17B, rmIL-17C, rmIL-17E or rmIL-17F.
- Purification
- Protein G purified
- Immunogène
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Recombinant protein corresponding to aa25-205 from mouse Interleukin 17D expressed in E. coli (NP_665836).
Type of Immunogen: Recombinant protein - Isotype
- IgG2a
- Top Product
- Discover our top product IL17D Anticorps primaire
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- Indications d'application
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Approved: ELISA, Flo, WB
Usage: Western Blot, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect mouse IL-17D. Using a colorimetric detection system, the detection limit for rmIL-17D is approximately 5 ng/lane under non-reducing and reducing conditions. Use of this antibody under non reducing/reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using CD4+ splenic lymphocytes. For intracellular staining to detect IL-17D, cells must first be fixed and permeabilized with 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse IL-17D. The detection limit for rmIL-17D is approximately 1 ng/well. - Commentaires
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- 200 μL sterile PBS
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, 5 % trehalose.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
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- Antigène
- IL17D (Interleukin 17D (IL17D))
- Autre désignation
- IL17D (IL17D Produits)
- Synonymes
- anticorps IL-17D, anticorps IL-22, anticorps IL-27, anticorps IL27, anticorps AI462269, anticorps Il27a, anticorps il17-4, anticorps zgc:194257, anticorps zgc:194303, anticorps IL-27p28, anticorps Il30, anticorps p28, anticorps interleukin 17D, anticorps interleukin 17d, anticorps interleukin 27, anticorps IL17D, anticorps Il17d, anticorps il17d, anticorps Il27
- Sujet
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Name/Gene ID: IL17D
Family: Interleukin
Synonyms: IL17D, IL-27, Interleukin 27, Interleukin-17D, Interleukin-27, IL-17D, IL-22, Interleukin 17D - ID gène
- 53342
- NCBI Accession
- NP_665836
- UniProt
- Q8TAD2
- Pathways
- Cellular Response to Molecule of Bacterial Origin
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