Actin anticorps (N-Term)

N° du produit ABIN331838

L’anticorps Souris Monoclonal anti-Actin a été validé pour WB, IHC, ELISA, IHC (p) et ICC. Il convient pour détecter Actin dans des échantillons de Humain, Rat, Souris, Poulet, Lapin, Cobaye et Mouton.

Aperçu rapide pour Actin anticorps (N-Term) (ABIN331838)

Antigène: Actin

Reactivité: Humain, Rat, Souris, Poulet, Lapin, Cobaye, Mouton

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Actin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Détail du produit anti-Actin anticorps

Épitope: AA 50-70, N-Term

Specificité: Recognizes all six isoforms of vertebrate actin, globular (G) and filamentous (F) forms. This clone is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. Labels myotubes and stains myoblasts and fibroblasts. Does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. Species cross-reactivity: All species.

Purification: Protein A purified

Immunogène: Purified chicken gizzard actin. Epitope: Appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70.

Type of Immunogen: Purified protein

Isotype: IgG2b kappa

Information d'application

Indications d'application: Approved: ELISA, ICC (1 - 20 μg/mL), IHC, IHC-P, WB

Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA. Immunohistochemistry: 10 μg/mL (paraffin embedded, 4 % formaldehyde, 3 % glutaraldehyde, sodium cacodylate treated sections). Visualized using a donkey anti-mouse IgG (Cy3). Positive staining pattern reflects both membrane and cytoplasmic staining. ELISA: Shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows significant binding to both Dictyostelium discoideum and Physarum polycephalum in ELISA.

Commentaires:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur Actin

Antigène: Actin