Actin anticorps

N° du produit ABIN331865

L’anticorps Souris Monoclonal anti-Actin a été validé pour WB, IHC, IHC (p) et IP. Il convient pour détecter Actin dans des échantillons de Humain, Rat, Souris, Poulet et Lapin.

Aperçu rapide pour Actin anticorps (ABIN331865)

Antigène: Actin

Reactivité: Humain, Rat, Souris, Poulet, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Actin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Détail du produit anti-Actin anticorps

Specificité: Reacts with all six known vertebrate isoforms of actin (MW ~42kD). It also reacts with two cytoplasmic actins (beta, gamma) which are highly homologous to one another, but which differ from the muscle actins by about 25 amino acid residues. This antibody is highly recommended for monitoring total protein load on Western blots. Species cross-reactivity: Human, bovine, mouse, canine, porcine, rabbit, rat, chicken, Dictyostelium discoideum, Physarum polycephalum.

Purification: Protein G purified

Immunogène: Chicken gizzard actin.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: IHC, IHC-P (0.5 μg/mL), IP, WB (0.5 μg/mL)

Usage: Suitable for use in Immunoprecipitation, Immunohistochemistry and Western Blot. Immunoprecipitation (Denatured verified): 2 μg/mg protein lysate. Use Protein G. Western Blot: 0.5 μg/mL for 2hrs at room temperature. Immunohistochemistry (formalin/paraffin): 1:400 for 30 min. Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Positive control: HeLa cells, skeletal muscle. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4.

Agent conservateur: Azide free

Détails sur Actin

Antigène: Actin