C4BP anticorps

N° du produit ABIN334259

L’anticorps anti-C4BP Monoclonal Souris est utilisé pour la détection de C4BP dans des échantillons de Humain. Il a été validé pour ELISA, IHC (p) et IHC.

Aperçu rapide pour C4BP anticorps (ABIN334259)

Antigène: C4BP (C4b-Binding Protein)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp C4BP est non-conjugé

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Clone: 10-07

Détail du produit anti-C4BP anticorps

Specificité: Recognizes complement component 4 binding protein (C4bp). There are three variants of C4bp, composed of alpha and beta chains. The predominant form is a 570 kD complex of 7 alpha chains and 1 beta chain. The other complexes formed are a 530 kD homoheptamer of alpha chains or a 500 kD complex of 6 alpha chains and 1 beta chain. C4bp regulates the activation of the classical complement pathway. It is a cofactor to the C3b/C4b inactivator, crucial to the proteolytic cleavage of C4b and important in cleavage of C3b. C4bp also regulates the C4bC2a complex (C3 convertase), accelerating its degradation by dissociating the complement fragment C2a. Both the C4bp alpha and beta chains interact with anticoagulant protein S and C4bp alpha binds protein S. C4bp is expressed in the blood serum.

Purification: Protein A purified from ascites

Immunogène: Native human C4 binding protein.

Isotype: IgG2b

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Borate buffered saline, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Store undiluted.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Détails sur C4BP

Antigène: C4BP (C4b-Binding Protein)

Autre désignation: C4BP / C4b-Binding Protein