Claudin 4 anticorps (C-Term)

N° du produit ABIN334265

L’anticorps Souris Monoclonal anti-Claudin 4 a été validé pour WB, IHC, IF et IHC (p). Il convient pour détecter Claudin 4 dans des échantillons de Humain, Rat et Chien.

Aperçu rapide pour Claudin 4 anticorps (C-Term) (ABIN334265)

Antigène: Claudin 4 (CLDN4)

Reactivité: Humain, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Claudin 4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Claudin 4 anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Recognizes the human ~22kD Claudin 4 protein and does not cross-react with the protein at 55kD. Species cross-reactivity: rat ileum homogenates and canine MDCK cell lysates by Western Blot and Immunofluorescence. Reactivity has also been confirmed with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by Immunohistochemistry.

Purification: Protein A purified

Immunogène: Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin 4.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Information d'application

Indications d'application: Approved: IF (1 - 3 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Aliquot to Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Détails sur Claudin 4

Antigène: Claudin 4 (CLDN4)

Autre désignation: CLDN4 / Claudin 4

Sujet: Name/Gene ID: CLDN4
Family: Claudin

Synonyms: CLDN4, Claudin-4, CPE-receptor, CPETR, CPETR1, Claudin 4, CPE-R, HCPE-R, WBSCR8, CPER

ID gène: 1364

UniProt: O14493

Pathways: Hepatitis C