EEF2 anticorps (pThr56)

N° du produit ABIN334266

Cet anticorps anti-EEF2 est un anticorps Lapin Polyclonal détectant EEF2 dans WB, IHC, ELISA et IHC (p). Adapté pour Humain, Souris, Rat, Poulet, Singe et Hamster.

Aperçu rapide pour EEF2 anticorps (pThr56) (ABIN334266)

Antigène: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Reactivité: Humain, Souris, Rat, Poulet, Singe, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EEF2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-EEF2 anticorps

Épitope: pThr56

Marque: IHC-plus™

Specificité: Detects endogenous levels of eEF2 only when phosphorylated at threonine 56. It does not recognize eEF2 phosphorylated at other sites.

Purification: Immunoaffinity purified

Immunogène: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr56 of human eEF2.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-P (1:100), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100. Western Blot: 1:1000, Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4uC with gentle shaking, overnight.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur EEF2

Antigène: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Autre désignation: EEF2 / Elongation Factor 2

Sujet: Name/Gene ID: EEF2

Synonyms: EEF2, EEF-2, EF-2, Elongation factor 2, Polypeptidyl-tRNA translocase, EF2

ID gène: 1938

UniProt: P13639

Pathways: AMPK Signaling