HNF1B anticorps (Internal Region)

N° du produit ABIN334505

L’anticorps Chèvre Polyclonal anti-HNF1B a été validé pour WB, ELISA et ICC. Il convient pour détecter HNF1B dans des échantillons de Humain et Souris. Il y a 1 publication disponible.

Aperçu rapide pour HNF1B anticorps (Internal Region) (ABIN334505)

Antigène: HNF1B (HNF1 Homeobox B (HNF1B))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp HNF1B est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Classe de qualité: Verified

Détail du produit anti-HNF1B anticorps

Épitope: Internal Region

Fonction: TCF2 / VHNF1

Séquence: QAYDRQKNPS KEER

Specificité: This antibody is expected to recognize both reported isoforms (NP_000449.1, NP_001159395.1). This product may cross-react with HNF1A.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat, Poisson zèbre (Danio rerio)

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QAYDRQKNPSKEER, from the internal region of the protein sequence according to NP_000449.1, NP_001159395.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 60 kDa band observed in Human Liver lysates (calculated MW of 61.3 kDa according to NP_000449.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. An additional band of unknown identity was also consistently ob

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunocytochemistry: A customer found positive results in ICC on Human primitive gut tube from Human embryonic stem cells.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-HNF1B (ABIN334505)

Arenas, Hervias, Uriz, Joplin, Prieto, Medina: "Combination of ursodeoxycholic acid and glucocorticoids upregulates the AE2 alternate promoter in human liver cells." dans: The Journal of clinical investigation, Vol. 118, Issue 2, pp. 695-709, (2008) (PubMed).

Détails sur HNF1B

Antigène: HNF1B (HNF1 Homeobox B (HNF1B))

Autre désignation: HNF1B

Sujet: TCF2, VHNF1, transcription factor 2, hepatic LF-B3 variant hepatic nuclear factor, FJHN, HNF1B, HNF1beta, HNF2, LFB3, MODY5, transcription factor 2

ID gène: 6928, 21410, 25640

NCBI Accession: NP_000449, NP_001159395

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation