COXII anticorps

N° du produit ABIN334535

L’anticorps Lapin Polyclonal anti-COXII a été validé pour WB et BN PAGE. Il convient pour détecter COXII dans des échantillons de Arabidopsis thaliana, Moss, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Wild Cabbage, Chickpea, Nicotiana tabacum et Zea mays. Il y a 4+ publications disponibles.

Aperçu rapide pour COXII anticorps (ABIN334535)

Antigène: COXII (Cytochrome Oxidase Subunit II (COXII))

Reactivité: Arabidopsis thaliana, Moss, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Wild Cabbage, Chickpea, Nicotiana tabacum, Zea mays

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp COXII est non-conjugé

Application: Western Blotting (WB), Blue-native PAGE (BN PAGE)

Détail du produit anti-COXII anticorps

Specificité: Arabidopsis thaliana (leaf extract and isolated mitochondria).

Homologie: dicots including Cucumis melo, Glycine max, Pisum sativum, monocots including Oryza sativa, moss Physcomitrella patens

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 29.4 / 30 kDa (for Arabidopsis thaliana)

Purification: serum

Immunogène: KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots includingArabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9

Information d'application

Indications d'application: Recommended Dilution 1 : 1000 (WB), 1 : 1000 (BN-PAGE).

Commentaires:

antibody detects COXII protein in total leaf extracts and isolated mitochondriaBlue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS pH 7.4

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Références pour anticorps anti-COXII (ABIN334535)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." dans: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Wang, Vanlerberghe: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." dans: Physiologia plantarum, (2013) (PubMed).

Hopff, Wienkoop, Lüthje: "The plasma membrane proteome of maize roots grown under low and high iron conditions." dans: Journal of proteomics, Vol. 91, pp. 605-18, (2013) (PubMed).

Miller, Grant, Giles, Ribas-Carbo, Berry, Watling, Robinson: "In the heat of the night - alternative pathway respiration drives thermogenesis in Philodendron bipinnatifidum." dans: The New phytologist, (2010) (PubMed).

Détails sur COXII

Antigène: COXII (Cytochrome Oxidase Subunit II (COXII))

Autre désignation: COXII

Sujet: AGI Code: ATMG00160
Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2

Poids moléculaire: expected: 29.4 kDa, apparent: 30 kDa (for Arabidopsis thaliana)

UniProt: Q1XGA9, P04373