COXII anticorps

N° du produit ABIN334535

Il existe 4+ publications pour ce produit. L’anticorps anti-COXII Polyclonal Lapin est utilisé pour la détection de COXII dans des échantillons de Cabbage, Physcomytrium Patens, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Arabidopsis thaliana, durum wheat, Zea mays, Vicia faba, Triticum aestivum, Rape, Quercus rubra, Picea abies, Nicotiana tabacum, Lilium longiflorum (Trumpet lily), Eriophorum vaginatum, Cucumis sativus, Cucumis melo, Chickpea, Betula nana, Orge et Actinidia chinensis. Il a été validé pour WB et BN PAGE.

Aperçu rapide pour COXII anticorps (ABIN334535)

Antigène: COXII (Cytochrome Oxidase Subunit II (COXII))

Reactivité: Cabbage, Physcomytrium Patens, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Arabidopsis thaliana, durum wheat, Zea mays, Vicia faba, Triticum aestivum, Rape, Quercus rubra, Picea abies, Nicotiana tabacum, Lilium longiflorum (Trumpet lily), Eriophorum vaginatum, Cucumis sativus, Cucumis melo, Chickpea, Betula nana, Orge, Actinidia chinensis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp COXII est non-conjugé

Application: Western Blotting (WB), Blue-native PAGE (BN PAGE)

Détail du produit anti-COXII anticorps

Fonction: Anti-COXII | Plant Cytochrome oxidase subunit II (affinity purified)

Specificité: Arabidopsis thaliana (leaf extract and isolated mitochondria).

Réactivité croisée (Details): Actinidia chinensis, Arabidopsis thaliana (leaf extract and isolated mitochondria), Betula nana, Brassica napus, Brassica oleracea, Cicer aretinum, Cucumis melo, Cucumis sativus, Erophorum vaginatum, Hordeum vulgare, Lilium longifolorum, Nicotiana tabacum, Picea abies, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica, Physcomitrium patens, Triticum aestivum, Triticum durum Desf., Zea mays, Vicia faba, Quercus rubra
Not reactive in: Mangifera indica, Saccharina japonica

Homologie: Cucumis melo,Glycine max,Gossypium hirsutum,Neurachne alopecuroidea,Neurachne minor,Neurachne muelleri,Oryza sativa,Physcomitrium patens,Pisum sativum,Triticum aestivum,Vigna radiata

Purification: Immunogen affinity purified serum

Immunogène: KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160

Information d'application

Indications d'application: 1 : 1000 (BN-PAGE), 1 : 1000 (WB)

Commentaires:

antibody detects COXII protein in total leaf extracts and isolated mitochondriaBlue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Références pour anticorps anti-COXII (ABIN334535)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." dans: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Wang, Vanlerberghe: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." dans: Physiologia plantarum, (2013) (PubMed).

Hopff, Wienkoop, Lüthje: "The plasma membrane proteome of maize roots grown under low and high iron conditions." dans: Journal of proteomics, Vol. 91, pp. 605-18, (2013) (PubMed).

Miller, Grant, Giles, Ribas-Carbo, Berry, Watling, Robinson: "In the heat of the night - alternative pathway respiration drives thermogenesis in Philodendron bipinnatifidum." dans: The New phytologist, (2010) (PubMed).

Détails sur COXII

Antigène: COXII (Cytochrome Oxidase Subunit II (COXII))

Autre désignation: COXII

Sujet:

Background: Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2

Additional information: Cellular [compartment marker] of mitochondrial inner membrane

Poids moléculaire: 29.4 | 30 kDa (for Arabidopsis thaliana)

ID gène: 3989052

UniProt: P04373, Q1XGA9