TOP2 anticorps (C-Term)

N° du produit ABIN334538

Il existe 1 publication pour ce produit. L’anticorps anti-TOP2 Polyclonal Lapin est utilisé pour la détection de TOP2 dans des échantillons de Arabidopsis thaliana et Vicia faba. Il a été validé pour WB et IL.

Aperçu rapide pour TOP2 anticorps (C-Term) (ABIN334538)

Antigène: TOP2 (DNA Topoisomerase II (TOP2))

Reactivité: Arabidopsis thaliana, Vicia faba

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TOP2 est non-conjugé

Application: Western Blotting (WB), Immunolocalization (IL)

Détail du produit anti-TOP2 anticorps

Épitope: C-Term

Fonction: Anti-TOP2 | DNA topoisomerase II

Specificité: Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis cells the antibody detects a 170 kDa protein exclusively in the nulear fraction.

Réactivité croisée (Details): Arabidopsis thaliana, Vicia faba
Not reactive in: Nicotiana tabacum

Homologie: Brassica rapa,Chlamydomonas reinhardtii,Chlorella vulgaris,Citrus clementina,Glycine max,Hordeum vulgare,Medicago truncatula,Oryza sativa,Ostreococcus tauri,Panicum italicum,Phaseolus vulgaris,Physcomitrium patens,Pinus sitcHensis,Populus trichocarpa,Solanum tuberosum,Sorghum bicolor,Triticum aestivum,Vitis vinifera,Volvox Caterii

Purification: Serum

Immunogène: The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890

Information d'application

Indications d'application: 1 : 500 (IL), 1 : 2000 (WB)

Commentaires:

Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Références pour anticorps anti-TOP2 (ABIN334538)

Xie, Lam: "Characterization of a DNA Topoisomerase II cDNA from Arabidopsis thaliana." dans: Plant physiology, Vol. 106, Issue 4, pp. 1701-2, (1995) (PubMed).

Détails sur TOP2

Antigène: TOP2 (DNA Topoisomerase II (TOP2))

Autre désignation: TOP2

Sujet:

Background: Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II

Additional information: Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis thaliana cells the antibody detects a 170 kDa protein exclusively in the nulear fraction (see picture).

Poids moléculaire: 164 | 170 kDa (Arabidopsis thaliana)

ID gène: 821972

UniProt: P30182

Pathways: Mitotic G1-G1/S Phases