CDJ1 anticorps (N-Term)

N° du produit ABIN334548

Détail du produit anti-CDJ1 anticorps

Antigène: CDJ1 (Chloroplast DnaJ Protein (CDJ1))

Épitope: N-Term

Reactivité: Chlamydomonas reinhardtii

Hôte: Lapin

Clonalité: Polyclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Réactivité croisée (Details): Not reactive in: higher plants

Homologie: Ostreococcus sp.

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 40 / 40 kDa

Purification: serum

Immunogène: fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli

Information d'application

Indications d'application: 1. 3000 with standard ECL (WB)

Commentaires:

western blot has been done on 10-50 µg load per well, immunoprecipitation has been done on 5-50 µl

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Références pour anticorps anti-CDJ1 (ABIN334548)

Long, Sommer, Allen, Lu, Merchant: "FER1 and FER2 encoding two ferritin complexes in Chlamydomonas reinhardtii chloroplasts are regulated by iron." dans: Genetics, Vol. 179, Issue 1, pp. 137-47, (2008) (PubMed).

Willmund, Dorn, Schulz-Raffelt, Schroda: "The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C." dans: Plant physiology, Vol. 148, Issue 4, pp. 2070-82, (2008) (PubMed).

Détails sur CDJ1

Antigène: CDJ1 (Chloroplast DnaJ Protein (CDJ1))

Autre désignation: CDJ1

Synonymes: anticorps chloroplast DnaJ-like protein, anticorps CDJ1

Sujet: CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.

Poids moléculaire: expected: 40 kDa, apparent: 40 kDa

UniProt: Q66YD3