50S Ribosomal Protein L30 anticorps

N° du produit ABIN7676204

Cet anticorps anti-50S Ribosomal Protein L30 Polyclonal Lapin (ABIN7676204) détecte spécifiquement 50S Ribosomal Protein L30 dans IF et WB. L’anticorps est réactif avec des échantillons de Chlamydomonas reinhardtii et Spinach.

Aperçu rapide pour 50S Ribosomal Protein L30 anticorps (ABIN7676204)

Antigène: 50S Ribosomal Protein L30

Reactivité: Chlamydomonas reinhardtii, Spinach

Hôte: Lapin

Clonalité: Polyclonal

Application: Immunofluorescence (IF), Western Blotting (WB)

Détail du produit

Fonction: Anti-L7/L12 | Ribosomal protein

Réactivité croisée (Details): Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Purification: Serum

Immunogène: Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece

Information d'application

Indications d'application: 1: 10 (IF), 1 : 1000 (WB)

Commentaires:

Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Détail du antigène

Antigène: 50S Ribosomal Protein L30

Autre désignation: 50S ribosomal protein L30 (L-30)

Sujet:

Background: Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96(5):1451-1463

Additional information: Name of this antibody has been changed from L-30 | 50S ribosomal protein L30 to L7/L12 based on the following reference: Randolph-Anderson et al. (1989). Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved. J Mol Evol. 1989 Jul,29(1):68-88.

Poids moléculaire: 11.9 kDa (Chlamydomonas reinhardtii), 15.7 kDa (spinach)