Cet anticorps anti-HSP70 est un anticorps Lapin Polyclonal détectant HSP70 dans WB et IP. Adapté pour Plasmodium falciparum, Arabidopsis thaliana, Zea mays, Tomato, Orge et Algae (Desmodesmus subspicatus). Ce Primary Antibody a été cité dans 4+ publications.
Expected / apparent Molecular Weight of the Antigene: 70 kDa Detection limit is 4 pg, 11 fmol, linear range of logit/log plot is 0.02-6 pmol,midrange (B/ B o =50 %) 112 pg, 300 fmol.
Purification
serum
Immunogène
KLH-conjugated synthetic peptide conserved in known higher plant HSC70 proteins including three isoforms of Arabidopsis thaliana HSC70-1 (NP_ 001119156.1), HSC70-2 (NP_195869.1) and HSC70-3 (NP_187555.1) as well as heat shock inducible Hsp70 of Arabidopsis thaliana At3g12580/T2E22_110 and At3g16030 and At3g12580/T2E22_110
Recommended Dilution: 1 : 3000 with standard ECL on 5 µg of protein per well (WB), 2-3 µl/protein extractof concentration 3-5 mg/mL.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 150 µL of sterile water.
Conseil sur la manipulation
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Stock
-20 °C
Norhayati, Hazlina, Asrenee, Emilin: "Magnitude and risk factors for postpartum symptoms: a literature review." dans: Journal of affective disorders, Vol. 175, pp. 34-52, (2015) (PubMed).
Muralidharan, Oksman, Pal, Lindquist, Goldberg: "Plasmodium falciparum heat shock protein 110 stabilizes the asparagine repeat-rich parasite proteome during malarial fevers." dans: Nature communications, Vol. 3, pp. 1310, (2012) (PubMed).
Alvarez, del Hoyo, García-Breijo, Reig-Armiñana, del Campo, Guéra, Barreno, Casano: "Different strategies to achieve Pb-tolerance by the two Trebouxia algae coexisting in the lichen Ramalina farinacea." dans: Journal of plant physiology, Vol. 169, Issue 18, pp. 1797-806, (2012) (PubMed).
Janicka-Russak, Kaba?a, Burzynski: "Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots." dans: Journal of experimental botany, Vol. 63, Issue 11, pp. 4133-42, (2012) (PubMed).
Antigène
HSP70
(Heat Shock Protein 70 (HSP70))
Autre désignation
HSP70
Sujet
AGI Code: At3g12580 Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.