KRT4 anticorps

N° du produit ABIN335335

Détail du produit anti-KRT4 anticorps

Antigène: KRT4 (Keratin 4 (KRT4))

Reactivité: Humain, Chien, Cat, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Human, canine and feline.

Purification: Purified

Immunogène: 6B10 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytokeratin preparation extracted from human esophagus.

Clone: 6B10

Isotype: IgG1

Information d'application

Indications d'application: 6B10 reacts exlcusively with cytokeratin 4 which is present in non-cornifying squamous epithelium, including cornea and transitional epithelium. Cells in certain ciliated pseudo-stratified epithelia and ductal epithelia of various exocrine glands are also positive for 6B10. 6B10 is useful for immunocytochemistry, immunohistochemistry on frozen and tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour anticorps anti-KRT4 (ABIN335335)

Corver, Koopman, van der Aa, Regensburg, Fleuren, Cornelisse: "Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer." dans: Cytometry, Vol. 39, Issue 2, pp. 96-107, (2000) (PubMed).

Vos, van den Ingh, de Neijs, van Mil, Ivanyi, Ramaekers: "Immunohistochemistry with keratin monoclonal antibodies in canine tissues: urogenital tract, respiratory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord." dans: Zentralblatt für Veterinärmedizin. Reihe A, Vol. 39, Issue 10, pp. 721-40, (1993) (PubMed).

Ivanyi, Minke, Hageman, Groeneveld, van Doornewaard, Misdorp: "Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas." dans: American journal of veterinary research, Vol. 54, Issue 7, pp. 1095-102, (1993) (PubMed).

Ivanyi, Minke, Hageman, Groeneveld, van Doornewaard: "Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells." dans: American journal of veterinary research, Vol. 53, Issue 3, pp. 304-14, (1992) (PubMed).

Weikel, Wagner, Moll: "Characterization of subcolumnar reserve cells and other epithelia of human uterine cervix. Demonstration of diverse cytokeratin polypeptides in reserve cells." dans: Virchows Archiv. B, Cell pathology including molecular pathology, Vol. 54, Issue 2, pp. 98-110, (1988) (PubMed).

van Muijen, Ruiter, Franke, Achtstätter, Haasnoot, Ponec, Warnaar: "Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13." dans: Experimental cell research, Vol. 162, Issue 1, pp. 97-113, (1986) (PubMed).

Détails sur KRT4

Antigène: KRT4 (Keratin 4 (KRT4))

Autre désignation: Cytokeratin 4 / Keratin K4 (KRT4 Produits)

Synonymes: anticorps cb59, anticorps etID20321.6, anticorps krt8, anticorps wu:fa91a11, anticorps wu:fd19e09, anticorps wu:fl23c09, anticorps zf-K8, anticorps CK-4, anticorps CK4, anticorps CYK4, anticorps K4, anticorps Kb4, anticorps AW108092, anticorps Krt-2.4, anticorps Krt2-4, anticorps keratin 4, anticorps krt4, anticorps KRT4, anticorps Krt4

Sujet: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.