Coat Protein g8p anticorps

N° du produit ABIN335342

Détail du produit

Antigène: Coat Protein g8p

Reactivité: Bacteriophage M13

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Affinity Chromatography (AC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Purification: Purified

Immunogène: RL-ph1 is a mouse monoclonal IgG2b, kappa antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with isolated M13 phage coat proteins.

Clone: RL-ph1

Isotype: IgG2b

Information d'application

Indications d'application: RL-ph1 reacts with the major M13 filamentous phage coat protein g8p with a molecular weight of 5 kDa. RL-ph1 is particularly useful for immunoblotting of separated phage proteins, and is also suitable for immunocytochemistry, flow cytometry, affinity chromatography and ELISA. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Référence

Meulemans, Nieland, Debie, Ramaekers, van Eys: "Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody." dans: Human antibodies and hybridomas, Vol. 6, Issue 3, pp. 113-8, (1996) (PubMed).

Détail du antigène

Antigène: Coat Protein g8p

Classe de substances: Phage Protein

Sujet: The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.