160 kDa Neurofilament anticorps

N° du produit ABIN335366

L’anticorps Souris Monoclonal anti- a été validé pour WB, IHC, IHC (fro) et IHC (p). Il convient pour détecter dans des échantillons de Humain, Hamster, Singe et Xenopus laevis. Il y a 1 publication disponible.

Aperçu rapide pour 160 kDa Neurofilament anticorps (ABIN335366)

Antigène: 160 kDa Neurofilament

Reactivité: Humain, Hamster, Singe, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RNF403

Détail du produit

Specificité: Human, hamster, monkey and Xenopus.

Purification: Purified

Immunogène: RNF403 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a neurofilament preparation of calf brain tissue.

Isotype: IgG1

Information d'application

Indications d'application: RNF403 reacts exclusively with the phosphorylated isoform of the160 kD neurofilament protein. RNF403 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:50 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Référence

Bauwens, De Groot, Ramaekers, Veldman, Huizing: "Expression of intermediate filament proteins in the adult human vestibular labyrinth." dans: The Annals of otology, rhinology, and laryngology, Vol. 101, Issue 6, pp. 479-86, (1992) (PubMed).

Détail du antigène

Antigène: 160 kDa Neurofilament

Autre désignation: Neurofilament 160 kDa

Sujet: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.