160 kDa Neurofilament anticorps

N° du produit ABIN335367

Cet anticorps anti- est un anticorps Souris Monoclonal détectant dans WB, IHC, IHC (fro) et IHC (p). Adapté pour Humain, Hamster, Singe, Xenopus laevis, Cobaye et Lapin.

Aperçu rapide pour 160 kDa Neurofilament anticorps (ABIN335367)

Antigène: 160 kDa Neurofilament

Reactivité: Humain, Hamster, Singe, Xenopus laevis, Cobaye, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RNF406

Détail du produit

Specificité: Human, rabbit, hamster, monkey, guinea pig and Xenopus.

Purification: Purified

Immunogène: RNF406 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a neurofilament preparation of calf brain tissue.

Isotype: IgG1

Information d'application

Indications d'application: RNF406 reacts exclusively with the phosphorylated isoform of the160 kD neurofilament protein. RNF406 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:50 - 1:100 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: 160 kDa Neurofilament

Autre désignation: Neurofilament 160 kDa

Sujet: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.