Vimentin anticorps

N° du produit ABIN335381

L’anticorps Souris Monoclonal anti-Vimentin a été validé pour WB, IHC, FACS, ICC et IHC (fro). Il convient pour détecter Vimentin dans des échantillons de Humain, Rat, Porc, Chien, Chévre et Hamster. Il y a 1 publication disponible.

Aperçu rapide pour Vimentin anticorps (ABIN335381)

Antigène: Vimentin (VIM)

Reactivité: Humain, Rat, Porc, Chien, Chévre, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RV203

Détail du produit anti-Vimentin anticorps

Specificité: Human, rat, goat, canine, hamster and swine.

Purification: Purified

Immunogène: RV203 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Isotype: IgG1

Information d'application

Indications d'application: RV203 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV203 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour anticorps anti-Vimentin (ABIN335381)

Schaart, Pieper, Kuijpers, Bloemendal, Ramaekers: "Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins." dans: Differentiation; research in biological diversity, Vol. 46, Issue 2, pp. 105-15, (1991) (PubMed).

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

Sujet: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

Pathways: Caspase Cascade in Apoptosis