Vimentin anticorps

N° du produit ABIN335382

Cet anticorps anti-Vimentin est un anticorps Souris Monoclonal détectant Vimentin dans WB, IHC, FACS, IHC (p), ICC et IHC (fro). Adapté pour Humain, Rat, Poulet et Potoroo. Ce Primary Antibody a été cité dans 6+ publications.

Aperçu rapide pour Vimentin anticorps (ABIN335382)

Antigène: Vimentin (VIM)

Reactivité: Humain, Rat, Poulet, Potoroo

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: V9

Détail du produit anti-Vimentin anticorps

Specificité: Human, rat, chicken and potoroo.

Purification: Purified

Immunogène: V9 is a mouse monoclonal IgG1 antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with vimentin isolated from porcine lens.

Isotype: IgG1

Information d'application

Indications d'application: V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. V9 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer (pH 6.0) is required. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour anticorps anti-Vimentin (ABIN335382)

De Visscher, Plusquin, Mesure, Flameng: "Selection of an immunohistochemical panel for cardiovascular research in sheep." dans: Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry, Vol. 18, Issue 4, pp. 382-91, (2010) (PubMed).

Corver, Koopman, van der Aa, Regensburg, Fleuren, Cornelisse: "Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer." dans: Cytometry, Vol. 39, Issue 2, pp. 96-107, (2000) (PubMed).

Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, (1987) (PubMed).

Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." dans: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, (1987) (PubMed).

Tölle, Weber, Osborn: "Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments." dans: European journal of cell biology, Vol. 38, Issue 2, pp. 234-44, (1985) (PubMed).

Osborn, Debus, Weber: "Monoclonal antibodies specific for vimentin." dans: European journal of cell biology, Vol. 34, Issue 1, pp. 137-43, (1984) (PubMed).

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

Sujet: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.

Pathways: Caspase Cascade in Apoptosis