Selectin E/CD62e anticorps

N° du produit ABIN335384

L’anticorps Rat Monoclonal anti-Selectin E/CD62e a été validé pour WB, IHC, ELISA, IP, FACS, ICC, IHC (fro) et Func. Il convient pour détecter Selectin E/CD62e dans des échantillons de Souris. Il y a 2+ publications disponibles.

Aperçu rapide pour Selectin E/CD62e anticorps (ABIN335384)

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Selectin E/CD62e est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: UZ5

Détail du produit anti-Selectin E/CD62e anticorps

Specificité: Mouse.

Purification: Purified

Immunogène: UZ5 is a rat monoclonal IgG2b antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a Lewis rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface.

Isotype: IgG2b

Information d'application

Indications d'application: UZ5 recognizes a conformational epitope in the CR1 region in murine E-selectin. UZ5 partially inhibits leukocyte adhesion to murine activated endothelial cells. UZ5 is suitable for immunoblotting, ELISA, immunoprecipitation, immunohistochemistry on fozen sections and flow cytometry (dilution 1:200).

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour anticorps anti-Selectin E/CD62e (ABIN335384)

Hammel, Weitz-Schmidt, Krause, Moll, Vestweber, Zerwes, Hallmann: "Species-specific and conserved epitopes on mouse and human E-selectin important for leukocyte adhesion." dans: Experimental cell research, Vol. 269, Issue 2, pp. 266-74, (2001) (PubMed).

Hallmann, Zimmermann, Sorokin, Needham, Von der Mark: "Adhesion of leukocytes to the inflammed endothelium." dans: Scandinavian journal of rheumatology. Supplement, Vol. 101, pp. 107-9, (1995) (PubMed).

Détails sur Selectin E/CD62e

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Autre désignation: E-selectin

Sujet: Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkappaB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

Pathways: Thromboxane A2 Receptor Signaling