Reticulon 1A anticorps

N° du produit ABIN335394

Cité dans 5 publications. Cet anticorps anti-Reticulon 1A Monoclonal Souris (Clone MON160) (ABIN335394) détecte spécifiquement Reticulon 1A dans IHC (fro), IHC (p), IHC, WB, FACS et ICC. L’anticorps est réactif avec des échantillons de Humain, Hamster, Souris et Rat.

Aperçu rapide pour Reticulon 1A anticorps (ABIN335394)

Antigène: Reticulon 1A

Reactivité: Humain, Hamster, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Reticulon 1A est non-conjugé

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: MON160

Détail du produit anti-Reticulon 1A anticorps

Specificité: Human, mouse, hamster and rat.

Purification: Purified

Immunogène: MON-160 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with a partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776 ).

Isotype: IgG1

Information d'application

Indications d'application: MON-160 exclusively recognizes the 135 kD Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues. The epitope of MON-160 is located between amino acid residues 174-337 of Reticulon-1A. MON-160 is useful for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissue, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:50 - 1:100 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:50 - 1:500 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour anticorps anti-Reticulon 1A (ABIN335394)

Senden, Timmer, de Bruïne, Wagenaar, Van de Velde, Roebroek, Van de Ven, Broers, Ramaekers: "A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers." dans: The Journal of pathology, Vol. 182, Issue 1, pp. 13-21, (1997) (PubMed).

van de Velde, Roebroek, Senden, Ramaekers, Van de Ven: "NSP-encoded reticulons, neuroendocrine proteins of a novel gene family associated with membranes of the endoplasmic reticulum." dans: Journal of cell science, Vol. 107 ( Pt 9), pp. 2403-16, (1995) (PubMed).

van de Velde, Senden, Roskams, Broers, Ramaekers, Roebroek, Van de Ven: "NSP-encoded reticulons are neuroendocrine markers of a novel category in human lung cancer diagnosis." dans: Cancer research, Vol. 54, Issue 17, pp. 4769-76, (1994) (PubMed).

van de Velde, Roebroek, van Leeuwen, Van de Ven: "Molecular analysis of expression in rat brain of NSP-A, a novel neuroendocrine-specific protein of the endoplasmic reticulum." dans: Brain research. Molecular brain research, Vol. 23, Issue 1-2, pp. 81-92, (1994) (PubMed).

Roebroek, van de Velde, Van Bokhoven, Broers, Ramaekers, Van de Ven: "Cloning and expression of alternative transcripts of a novel neuroendocrine-specific gene and identification of its 135-kDa translational product." dans: The Journal of biological chemistry, Vol. 268, Issue 18, pp. 13439-47, (1993) (PubMed).

Détails sur Reticulon 1A

Antigène: Reticulon 1A

Autre désignation: Reticulon-1A / NSP-A

Sujet: Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).