Specific for immunohistochemical detection of 7KC in atherosclerotic aorta.
Purification
Protein A purified
Clone
35A
Isotype
IgG
Indications d'application
It is recommended that the antibody be tried at 5-10 µg/mL on frozen tissue. Not applicable to paraffin embedded tissues.
Restrictions
For Research Use only
Format
Frozen
Concentration
1 mg/mL
Buffer
10 mM PBS containing 0.1 % NaN3 and 0.1 % BSA
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Moreira, Larrayoz, Lee, Rodríguez: "7-Ketocholesterol is present in lipid deposits in the primate retina: potential implication in the induction of VEGF and CNV formation." dans: Investigative ophthalmology & visual science, Vol. 50, Issue 2, pp. 523-32, (2009) (PubMed).
Rodriguez, Fliesler: "Photodamage generates 7-keto- and 7-hydroxycholesterol in the rat retina via a free radical-mediated mechanism." dans: Photochemistry and photobiology, Vol. 85, Issue 5, pp. 1116-25, (2009) (PubMed).
Antigène
7KC-Modified Keyhole-Lympet Hemocyanine
Classe de substances
Chemical
Sujet
About 7-ketocholesterol (7-KC) Cholesterol oxidation products, especially 7-Ketocholesterol (7KC), have been the focus of much attention because they are present in human atherosclerotic plaque and display a wide range of atherogenic properties in vitro and to some extant in vivo. This antibody is specific for 7KC. This antibody is useful to detect 7KC in tissue samples. A frozen tissue sample is recommended for immunohistochemistry.