S1PR3 anticorps (AA 12-25)

N° du produit ABIN337001

L’anticorps Lapin Polyclonal anti-S1PR3 a été validé pour WB, IHC, IHC (p) et ICC. Il convient pour détecter S1PR3 dans des échantillons de Humain.

Aperçu rapide pour S1PR3 anticorps (AA 12-25) (ABIN337001)

Antigène: S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp S1PR3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Détail du produit anti-S1PR3 anticorps

Épitope: AA 12-25

Marque: IHC-plus™

Specificité: Recognizes human EDG 3 receptor at ~47kD in human cerebral cortex. Liver and heart tissues display 2-3 bands between 40-50kD, possibly due to different degrees of glycosylation.

Homologie: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon (93%) Marmoset (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide corresponding to aa12-25 [VRGNETLREHYQYV] of human EDG 3 protein. Species sequence Homology: Mouse, rat: 79% (11/14). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon (93%), Marmoset (86%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: ICC, IHC, IHC-P (10 μg/mL), WB (3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: TBS,  pH 7.4, 5 mg/mL BSA

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur S1PR3

Antigène: S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

Autre désignation: SIPR3 / EDG3 / S1P3

Sujet: Name/Gene ID: S1PR3
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR

Synonyms: S1PR3, EDG-3, EDG3, LPB3, S1P receptor EDG3, S1P3, S1P receptor Edg-3, S1P receptor 3

ID gène: 1903

UniProt: Q99500