Midline 2 anticorps (AA 723-735)

N° du produit ABIN337007

L’anticorps anti-Midline 2 Polyclonal Chèvre est utilisé pour la détection de Midline 2 dans des échantillons de Humain, Souris et Singe. Il a été validé pour WB, ELISA, IHC et IHC (p).

Aperçu rapide pour Midline 2 anticorps (AA 723-735) (ABIN337007)

Antigène: Midline 2 (MID2)

Reactivité: Humain, Souris, Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Midline 2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Midline 2 anticorps

Épitope: AA 723-735

Specificité: Human MID2. This antibody is expected to recognise both human isoforms of this protein.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%) Mouse, Rat, Dog, Bat, Elephant, Panda, Horse, Pig, Opossum, Turkey, Chicken (92%) Hamster, Rabbit, Zebrafish, Stickleback (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide QESPYVSGMKTCH from the C-terminus of human MID2 (NP_036348.2, NP_438112.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%), Mouse, Rat, Dog, Bat, Elephant, Panda, Horse, Pig, Opossum, Turkey, Chicken (92%), Hamster, Rabbit, Zebrafish, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Midline 2

Antigène: Midline 2 (MID2)

Autre désignation: TRIM1 / MID2

Sujet: Name/Gene ID: MID2

Synonyms: MID2, FXY2, Midin-2, Midline-2, Midin 2, Midline 2, Midline defect 2, RNF60, RING finger protein 60, TRIM1, Tripartite motif protein 1

ID gène: 11043

NCBI Accession: NP_036348, NP_438112

UniProt: Q9UJV3